Development and validation of a fluorimetric method to determine curcumin in lipid and polymeric nanocapsule suspensions
نویسندگان
چکیده
A simple, rapid, and sensitive fluorimetric method was developed and validated to quantify curcumin in lipid and polymeric nanocapsule suspensions, using acetonitrile as a solvent. The excitation and emission wavelengths were set at 397 nm and 508 nm, respectively. The calibration graph was linear from 0.1 to 0.6 mg/mL with a correlation coefficient of 0.9982. The detection and quantitation limits were 0.03 and 0.10 mg/mL, respectively. The validation results confirmed that the developed method is specific, linear, accurate, and precise for its intended use. The current method was successfully applied to the evaluation of curcumin content in lipid and polymeric nanocapsule suspensions during the early stage of formulation development.
منابع مشابه
Development and Optimization of Polymeric Self-Emulsifying Nanocapsules for Localized Drug Delivery: Design of Experiment Approach
The purpose of the present study was to formulate polymeric self-emulsifying curcumin nanocapsules with high encapsulation efficiency, good emulsification ability, and optimal globule size for localized targeting in the colon. Formulations were prepared using modified quasiemulsion solvent diffusion method. Concentration of formulation variables, namely, X1 (oil), X2 (polymeric emulsifier), and...
متن کاملP1: Dextran Curcumin Promotes Novel Object Recognition Memory in Rats after Ischemic Stroke
Ischemic stroke causes the depletion of energy and induces excitotoxicity and neuroinflammation in the brain that results from thrombotic blockage. Cerebral ischemia leads to many types of memory loss, including impairment of working, spatial and object recognition memoreis. Curcumin shows strong anti-oxidoinflammatory activities but it terapathics limited by its low solubility in water and cor...
متن کاملCurcumin Alleviates Streptozotocin-Induced Hyperalgesia in Diabetic Neuropathic Rat via Attenuation of Lipid Peroxidation and Inflammation
Background and Objective: Hyperalgesia is one of the marked clinical signs of patients with diabetes mellitus that affects their lifestyle. This study was conducted to assess the anti-nociceptive effect of chronic administration of curcumin in streptozotocin (STZ)-diabetic rats using formalin, hot tail immersion, and paw pressure tests. Materials and Methods: This study was conducted on 40 male...
متن کاملThe Effect of Combined Exercise Training Course with Curcumin Supplementation on Lipid Profiles of Inactive Middle-Aged Men
Objective: The purpose of this study was to investigate the effect of exercise training and curcumin supplementation combination on the lipid profile of inactive middle-aged men. Materials and Methods: This is an experimental study. The studied population was middle-aged men who were categorized as inactive. The mean (± standard deviation) age of participants was 34/82 (±6.54). They were 60 vo...
متن کاملEffect of Curcumin Supplementation on Fructosamine Level, Blood Lipids, Lipid Peroxidation and Hepatic Enzymes in Type 2 Diabetics
Objective: DM is a major medical problem in developing countries such as Iran. Blood sugar and lipids levels increase in diabetics exacerbates the onset of diabetes mellitus (DM) late complications. Researchers have focused on herbal medicines improve sugar and lipid levels against side decreasing effects and possible inefficacy of chemical drugs. This study has assessed the effect of curcumin ...
متن کامل